Part:BBa_K3631000:Design
Part A of Serotonin Whole Cell Biosensor
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 157
Illegal PstI site found at 565 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 157
Illegal PstI site found at 565 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 85
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 157
Illegal PstI site found at 565 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 157
Illegal PstI site found at 565
Illegal NgoMIV site found at 412
Illegal AgeI site found at 609 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The chosen promoter should facilitate our regulation and its expression in E. coli. 1. Rampioni G, Bertani I, Zennaro E, Polticelli F, Venturi V, Leoni L. The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter. J Bacteriol. 2006 Jan;188(2):815-9. doi: 10.1128/JB.188.2.815-819.2006. PMID: 16385073; PMCID: PMC1347304. 2. Knecht LD, O'Connor G, Mittal R, Liu XZ, Daftarian P, Deo SK, Daunert S. Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host. EBioMedicine. 2016 Jul;9:161-169. doi: 10.1016/j.ebiom.2016.05.037. Epub 2016 May 28. Erratum in: EBioMedicine. 2017 Sep;23 :195. Pasini, Patrizia [removed]. PMID: 27333040; PMCID: PMC4972532.
Source
It is cloned from lasR gene in Pseudomonas aeruginosa. Sequences was acquired from NCBI: https://www.ncbi.nlm.nih.gov/gene/881789.